7OPI

Structure of a minimal SF3B core in complex with the inactive modulator spliceostatin E (form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	104.535, 108.280, 253.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.430 - 3.100
R-factor	0.2299
Rwork	0.228
R-free	0.26940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ife
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.780	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.084	3.870
Rmeas	0.088	4.017
Rpim	0.024	1.069
Number of reflections	64378	4387
<I/σ(I)>	17.5	0.8
Completeness [%]	99.9	98.3
Redundancy	13.5	13.8
CC(1/2)	1.000	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.15	277.15	0.1 M Bis-tris propane pH=7.15, 0.2 M KSCN, PEG-3350 11% (w/v), Glycerol (v/v) 10%