7OP2
Chadox1/ Chimpanzee adenovirus Y25 fiber knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-17 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.427, 112.260, 98.605 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 74.040 - 1.590 |
| R-factor | 0.2248 |
| Rwork | 0.224 |
| R-free | 0.24410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.498 |
| Data reduction software | XDS |
| Data scaling software | DIALS |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 74.040 | 74.040 | 1.680 |
| High resolution limit [Å] | 1.590 | 5.030 | 1.590 |
| Rmerge | 0.288 | 0.094 | 3.342 |
| Rmeas | 0.300 | 0.097 | 3.476 |
| Rpim | 0.084 | 0.025 | 0.948 |
| Total number of observations | 3635051 | 133411 | 557114 |
| Number of reflections | 286462 | 9275 | 41679 |
| <I/σ(I)> | 5.7 | 19.7 | 0.8 |
| Completeness [%] | 100.0 | 100 | 99.9 |
| Redundancy | 12.7 | 14.4 | 13.4 |
| CC(1/2) | 0.996 | 0.998 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M CaCl2, 0.1 M MgCl2, 0.1 M PIPES pH7.0, 22.5 % v/v PEG Smear Medium and 0.2 M CaCl2, 0.1 M Tris pH8.0, 20 % w/v PEG 6000 |
