7OOG

Plasmodium falciparum Hsp70-x chaperone nucleotide binding domain in complex with NCL-00023823

Replaces:  7NQY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 21
Unit cell lengths	79.997, 101.027, 103.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.190 - 2.420
R-factor	0.2099
Rwork	0.209
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rzq
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (24-FEB-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.030	2.460
High resolution limit [Å]	2.420	2.420
Rmeas	0.117	2.063
Number of reflections	32637	1611
<I/σ(I)>	8.5	0.9
Completeness [%]	100.0
Redundancy	6.4
CC(1/2)	1.000	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	20 mM HEPES pH 7.4 50 mM NaCl 1 mM DTT 3.5 mM AMP-PNP 24% w/v PEG 1500 20% v/v glycerol