7ON5

Crystal structure of the SARS-CoV-2 neutralizing nanobody Re5D06

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	44.611, 44.611, 144.155
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.620 - 1.250
R-factor	0.147
Rwork	0.146
R-free	0.16680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7olz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.620	1.295
High resolution limit [Å]	1.250	1.250
Rmeas	0.056	0.411
Rpim	0.011	0.105
Number of reflections	41182	3846
<I/σ(I)>	38.44	7.6
Completeness [%]	99.4	95.13
Redundancy	23.8	14.8
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	100 mM MES: pH 6.0, 200 mM zinc acetate, 15% (v/v) ethanol