7ON3
SaFtsZ complexed with GDP (soak 10 mM EGTA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.858, 50.954, 88.488 |
Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
Resolution | 41.310 - 2.320 |
R-factor | 0.2367 |
Rwork | 0.234 |
R-free | 0.28820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.001 |
RMSD bond angle | 0.430 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.310 | 2.400 |
High resolution limit [Å] | 2.320 | 2.320 |
Number of reflections | 12638 | 1114 |
<I/σ(I)> | 6.94 | 1.77 |
Completeness [%] | 96.5 | |
Redundancy | 3.1 | |
CC(1/2) | 0.986 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 26% polyethylene glycol 5000 monomethyl ether (+ 10 mM EGTA for soaking, 23 h) |