7ON2

SaFtsZ complexed with GDP (soaking in 10 mM CyDTA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	C 1 2 1
Unit cell lengths	72.402, 50.207, 88.050
Unit cell angles	90.00, 111.42, 90.00

Refinement procedure

Resolution	40.980 - 1.690
R-factor	0.2061
Rwork	0.204
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.011
RMSD bond angle	1.215
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.980	1.750
High resolution limit [Å]	1.690	1.690
Number of reflections	31990	2889
<I/σ(I)>	13.23	1.97
Completeness [%]	96.5
Redundancy	3.2
CC(1/2)	0.998	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 26% polyethylene glycol 5000 monomethyl ether (+ 10 mM CyDTA for soaking, 23 h)