7OMQ
SaFtsZ complexed with GDPPCP and Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.890, 52.055, 87.937 |
Unit cell angles | 90.00, 110.34, 90.00 |
Refinement procedure
Resolution | 40.980 - 1.450 |
R-factor | 0.1624 |
Rwork | 0.161 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.147 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.980 | 1.500 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 52628 | 5227 |
<I/σ(I)> | 9.88 | 1.11 |
Completeness [%] | 98.7 | |
Redundancy | 4.4 | |
CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.5, and 22% polyethylene glycol 5000 monomethyl ether (+ 20 mM MnCl2 for soaking, 23h) |