7OMP
SaFtsZ complexed with GDPPCP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.583, 51.949, 87.779 |
Unit cell angles | 90.00, 110.11, 90.00 |
Refinement procedure
Resolution | 41.220 - 1.520 |
R-factor | 0.153 |
Rwork | 0.152 |
R-free | 0.17670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.169 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.220 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Number of reflections | 45499 | 4545 |
<I/σ(I)> | 14.62 | 1.82 |
Completeness [%] | 98.7 | |
Redundancy | 4.5 | |
CC(1/2) | 0.999 | 0.792 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.8, and 22% polyethylene glycol 5000 monomethyl ether (+20 mM MgCl2 for soaking, 23 h) |