7OMN

Anti-EphA1 JD1-1 VH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-07
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.000030
Spacegroup name	P 64
Unit cell lengths	131.870, 131.870, 49.679
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.839 - 1.700
R-factor	0.2038
Rwork	0.203
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b9v
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.839	19.839	1.800
High resolution limit [Å]	1.700	4.960	1.700
Rmerge	0.066	0.031	0.736
Rmeas	0.068	0.032	0.811
Total number of observations	810118
Number of reflections	51573	2260	7068
<I/σ(I)>	28.98	90.32	1.91
Completeness [%]	94.5	98.1	81.3
Redundancy	15.708	18.844	5.601
CC(1/2)	1.000	1.000	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M NaCl, 1 M K2HPO4 and 0.1 M Imidazole (pH 7)