7OMJ
SaFtsZ complexed with GDPPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.054, 51.861, 87.659 |
Unit cell angles | 90.00, 110.26, 90.00 |
Refinement procedure
Resolution | 41.120 - 1.570 |
R-factor | 0.1474 |
Rwork | 0.145 |
R-free | 0.18590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.125 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.120 | 1.620 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 41443 | 4104 |
<I/σ(I)> | 12.03 | 1.52 |
Completeness [%] | 98.8 | |
Redundancy | 4.1 | |
CC(1/2) | 0.998 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.7, and 22% polyethylene glycol 5000 monomethyl ether |