7OMA
Thosea asigna virus RdRP domain elongation complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.794, 207.305, 115.980 |
Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
Resolution | 67.780 - 3.100 |
R-factor | 0.2322 |
Rwork | 0.231 |
R-free | 0.25790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xhi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.858 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.780 | 3.211 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.384 | 2.066 |
Rpim | 0.842 | |
Number of reflections | 57906 | 5770 |
<I/σ(I)> | 6.79 | 1.22 |
Completeness [%] | 99.9 | 99.84 |
Redundancy | 6.9 | |
CC(1/2) | 0.951 | 0.391 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium acetate pH4.6, 0.22M (NH4)SO4, 28% PEG2000MME |