7OM6

Thosea asigna virus RdRP domain in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895
Spacegroup name	P 1 21 1
Unit cell lengths	65.636, 204.028, 70.076
Unit cell angles	90.00, 97.19, 90.00

Refinement procedure

Resolution	49.300 - 2.180
R-factor	0.2293
Rwork	0.229
R-free	0.24120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhi
RMSD bond length	0.012
RMSD bond angle	1.642
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.260
High resolution limit [Å]	2.180	2.180
Number of reflections	94404	9474
<I/σ(I)>	14.15
Completeness [%]	99.0
Redundancy	3.5
CC(1/2)	0.997	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M sodium acetate pH4.6, 0.25M (NH4)SO4, 26% PEG4000