7OLZ
Crystal structure of the SARS-CoV-2 RBD with neutralizing-VHHs Re5D06 and Re9F06
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.955, 57.017, 144.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.010 - 1.750 |
R-factor | 0.1775 |
Rwork | 0.176 |
R-free | 0.20920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yz5 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.010 | 1.813 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.090 | 1.530 |
Rmeas | 0.094 | 1.590 |
Rpim | 0.026 | 0.430 |
Number of reflections | 45701 | 2363 |
<I/σ(I)> | 17.91 | 1.99 |
Completeness [%] | 85.0 | 51.39 |
Redundancy | 13.3 | 13 |
CC(1/2) | 0.999 | 0.873 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M MOPS pH 7.5, 2.07 M ammonium sulfate, 0.1 M NDSB-256 |