7OL9
Crystal structure of C-terminally truncated Bacillus subtilis nucleoid occlusion protein (Noc) complexed to the Noc-binding site (NBS)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-21 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.501, 99.326, 99.416 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.360 - 2.900 |
R-factor | 0.2322 |
Rwork | 0.230 |
R-free | 0.27750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6y93 |
RMSD bond length | 0.003 |
RMSD bond angle | 1.183 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.360 | 70.360 | 3.070 |
High resolution limit [Å] | 2.890 | 8.670 | 2.890 |
Rmerge | 0.113 | 0.037 | 2.693 |
Rmeas | 0.118 | 0.039 | 2.797 |
Rpim | 0.032 | 0.012 | 0.752 |
Number of reflections | 16242 | 686 | 2578 |
<I/σ(I)> | 11.6 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 13.1 | 11.1 | 13.7 |
CC(1/2) | 0.999 | 1.000 | 0.684 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Null |