7OL2

Crystal structure of mouse contactin 1 immunoglobulin domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	92.959, 134.162, 181.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	107.940 - 3.890
R-factor	0.2223
Rwork	0.220
R-free	0.26950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2om5
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.090	4.270
High resolution limit [Å]	3.890	3.890
Number of reflections	20221	3909
<I/σ(I)>	4.4	1.5
Completeness [%]	93.9
Redundancy	9.3
CC(1/2)	0.961	0.524

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	277.15	20% w/v PEG 3350 and 0.2 M Magnesium nitrate hexahydrate pH 5.9