7OKR
Catalytic domain from the Aliivibrio salmonicida lytic polysaccharide monooxygenase AsLPMO10B
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 65 |
Unit cell lengths | 71.003, 71.003, 100.203 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.460 - 1.350 |
Rwork | 0.139 |
R-free | 0.16240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yn2 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.862 |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190806) |
Data scaling software | XSCALE (VERSION Mar 15, 2019 BUILT=20190806) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.460 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rpim | 0.022 | 0.585 |
Number of reflections | 62730 | 6133 |
<I/σ(I)> | 15.3 | 0.9 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 18.2 | 9.7 |
CC(1/2) | 0.999 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293.15 | 0.1 M phosphate/citrate pH 4.2, 40 % PEG 300 |