7OK7

Crystal structure of the UNC119B ARL3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	110.270, 152.190, 171.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.820 - 3.150
R-factor	0.2239
Rwork	0.221
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4goj
RMSD bond length	0.003
RMSD bond angle	0.712
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.820	3.230
High resolution limit [Å]	3.150	3.150
Number of reflections	50733	3702
<I/σ(I)>	0.114
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.998	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		279	1.00 M Ammonium di-hydrogen phosphate, 0.1M Tris PH7.5