7OK6

Crystal structure of UNC119B in complex with LCK peptide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91589
Spacegroup name	P 21 21 21
Unit cell lengths	55.083, 67.004, 106.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.742 - 1.949
Rwork	0.181
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h6a
RMSD bond length	0.010
RMSD bond angle	1.594
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.742	2.000
High resolution limit [Å]	1.949	1.949
Number of reflections	29562	2145
<I/σ(I)>	12.7
Completeness [%]	99.7
Redundancy	6.4
CC(1/2)	0.998	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	30 % w/v PEG 4,000, 0.1 M HEPES pH 7.5, 0.2 M CaCl2