7OK5

Crystal structure of mouse neurofascin 155 immunoglobulin domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	77.604, 96.273, 238.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.920 - 2.970
R-factor	0.2238
Rwork	0.221
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p3y
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.540	3.020
High resolution limit [Å]	2.970	2.970
Number of reflections	37686	1838
<I/σ(I)>	6.2	1
Completeness [%]	99.7	97.4
Redundancy	6.8	6.1
CC(1/2)	0.976	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277.15	20% (w/v) PEG 8000 and 100 mM HEPES/Sodium hydroxide pH 7.5