7OJS
Complex structure 2 of the Bacillus subtilis CdaA c-di-AMP cyclase domain (CdaACD) and the phosphoglucomutase GlmM short variant (GlmMF369)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.805, 228.536, 153.194 |
| Unit cell angles | 90.00, 99.86, 90.00 |
Refinement procedure
| Resolution | 75.466 - 4.200 |
| R-factor | 0.2294 |
| Rwork | 0.226 |
| R-free | 0.29550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Dimers of Bacillus subtilis CdaACD and GlmMF369 structures |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.180 | 75.460 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rpim | 0.062 | 0.223 |
| Number of reflections | 31005 | 30768 |
| <I/σ(I)> | 2.1 | 0.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.780 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M carboxylic acids, 0.1 M buffer system 1 (Imidazole; MES, pH 6.5) and 30% GOL_P4K (60% glycerol, PEG 4K) |
