7OJE
Crystal structure of the covalent complex between Tribolium castaneum deubiquitinase ZUP and Ubiquitin-PA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-19 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000021 |
Spacegroup name | P 61 |
Unit cell lengths | 151.607, 151.607, 83.751 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.670 - 2.050 |
R-factor | 0.1862 |
Rwork | 0.185 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ei1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.787 |
Data reduction software | XDS (20180126) |
Data scaling software | XDS (20180126) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.670 | 2.123 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 67359 | 6329 |
<I/σ(I)> | 12.83 | |
Completeness [%] | 97.9 | |
Redundancy | 8.6 | |
CC(1/2) | 0.998 | 0.411 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8 % (v/v) Tacsimate pH 7; 22 %PEG3350 |