7OJD
SaFtsZ(D46A) complexed with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 68.079, 53.898, 84.853 |
Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
Resolution | 41.500 - 1.820 |
R-factor | 0.1856 |
Rwork | 0.183 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.011 |
RMSD bond angle | 1.153 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.500 | 1.880 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 26341 | 8750 |
<I/σ(I)> | 12.85 | 1.44 |
Completeness [%] | 97.8 | |
Redundancy | 3.3 | |
CC(1/2) | 0.997 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.6, and 26% polyethylene glycol 5000 monomethyl ether |