7OJA
SaFtsZ(D210N) complexed with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 67.000, 53.194, 84.078 |
Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
Resolution | 41.030 - 2.220 |
R-factor | 0.1856 |
Rwork | 0.183 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.003 |
RMSD bond angle | 0.612 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.030 | 2.300 |
High resolution limit [Å] | 2.220 | 2.220 |
Number of reflections | 14229 | 1375 |
<I/σ(I)> | 9.47 | 1.77 |
Completeness [%] | 99.5 | |
Redundancy | 3.4 | |
CC(1/2) | 0.996 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.6, and 26% polyethylene glycol 5000 monomethyl ether |