Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.298, 73.298, 196.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.060 - 1.828 |
| Rwork | 0.181 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6adw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.934 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.060 | 1.940 |
| High resolution limit [Å] | 1.828 | 1.830 |
| Number of reflections | 48205 | 7581 |
| <I/σ(I)> | 2.22 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis Tris Propane pH 6.5, 0.2 M Sodium acetate trihydrate, 20% w/v PEG 3350, 10% v/v Ethylene glycol |
