7OIT

Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (P3221 crystal form)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	P 32 2 1
Unit cell lengths	66.364, 66.364, 161.556
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.470 - 1.650
Rwork	0.183
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phaser
RMSD bond length	0.013
RMSD bond angle	1.801
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.470	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.082	2.696
Rmeas		2.779
Rpim	0.019	0.668
Number of reflections	50587	2463
<I/σ(I)>	19	1.1
Completeness [%]	100.0	100
Redundancy	19.2	17

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	20mM Sodium formate; 20mM Ammonium acetate; 20mM Sodium citrate tribasic dihydrate; 20mM Sodium potassium tartrate tetrahydrate; 20mM Sodium oxamate, 100mM Imidazole MES monohydrate pH6.5 , 20% v/v Glycerol; 10% w/v PEG 4000