7OIM

Mouse RNF213, with mixed nucleotides bound

EM specimen

Vitrification	Yes
Instrument	LEICA PLUNGER

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K3 BIOQUANTUM (6k x 4k),GATAN K2 QUANTUM (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	4
Software	SPHIRE,RELION,PHENIX,SerialEM,CTFFIND,UCSF Chimera
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION