7OI5

Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91589
Spacegroup name	C 1 2 1
Unit cell lengths	116.046, 55.380, 167.735
Unit cell angles	90.00, 109.05, 90.00

Refinement procedure

Resolution	79.270 - 2.610
R-factor	0.2414
Rwork	0.238
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phaser
RMSD bond length	0.004
RMSD bond angle	0.728
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.270	2.650
High resolution limit [Å]	2.610	2.610
Rmerge	0.163	1.576
Rmeas	0.177	1.725
Rpim	0.068	0.693
Number of reflections	31089	1543
<I/σ(I)>	6.6	1
Completeness [%]	99.9	99.3
Redundancy	6.6
CC(1/2)	0.995	0.374

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.1	289	20% w/v PEG 3350, 0.2 M Sodium phosphate dibasic dehydrate pH 9.1. The crystal was cryo-protected by soaking in mother liquor supplemented with 25% glycerol.