7OI2

SaFtsz complexed with GDP (NaCl purification)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	69.187, 53.754, 85.349
Unit cell angles	90.00, 108.21, 90.00

Refinement procedure

Resolution	41.610 - 1.900
R-factor	0.1751
Rwork	0.172
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.007
RMSD bond angle	0.940
Data reduction software	XDS (1.18.2_3874)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.610	1.960
High resolution limit [Å]	1.900	1.900
Number of reflections	23465	2315
<I/σ(I)>	14.4	1.72
Completeness [%]	99.3
Redundancy	3.4
CC(1/2)	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8, 25% PEG5000 MME