7OHZ

Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-01
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97953
Spacegroup name	P 1 21 1
Unit cell lengths	55.683, 129.861, 64.423
Unit cell angles	90.00, 102.47, 90.00

Refinement procedure

Resolution	62.900 - 2.270
R-factor	0.2695
Rwork	0.267
R-free	0.31390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Phaser
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.900	2.310
High resolution limit [Å]	2.270	2.270
Rmerge	0.136	1.165
Rmeas	0.148	1.318
Rpim	0.058	0.593
Number of reflections	40099	1520
<I/σ(I)>	7	1.2
Completeness [%]	97.1	72.4
Redundancy	6.3	4.5
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	20% w/v PEG 3,350 0.2 M DL-Malic acid pH 7.0. The crystals were cryo-protected by soaking in mother liquor supplemented with 30-32% glycerol.