7OHN
SaFtsZ complexed with GDP, AlF4- and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9787 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.141, 52.006, 87.492 |
Unit cell angles | 90.00, 109.79, 90.00 |
Refinement procedure
Resolution | 41.160 - 1.620 |
R-factor | 0.1619 |
Rwork | 0.160 |
R-free | 0.20140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.099 |
Data reduction software | XDS (1.18.2_3874) |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.160 | 1.680 |
High resolution limit [Å] | 1.620 | 1.620 |
Number of reflections | 37431 | 3590 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.2 | |
Redundancy | 3.3 | |
CC(1/2) | 0.996 | 0.797 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris-HCl pH 8.6, 24% PEG 5000 MME |