7OHK

SaFtsZ complexed with GDP, BeF3- and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	C 1 2 1
Unit cell lengths	70.583, 52.276, 87.662
Unit cell angles	90.00, 110.05, 90.00

Refinement procedure

Resolution	41.180 - 1.750
R-factor	0.1617
Rwork	0.160
R-free	0.20020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rvn
RMSD bond length	0.009
RMSD bond angle	1.180
Data reduction software	XDS (1.18.2_3874)
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.180	1.813
High resolution limit [Å]	1.750	1.750
Number of reflections	29850	2895
<I/σ(I)>	10.02	1.73
Completeness [%]	98.1
Redundancy	2
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris-HCl pH 8.6, 25% PEG 5000 MME