7OGO

Plant peptide hormone receptor H1I1S1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999990
Spacegroup name	P 21 21 21
Unit cell lengths	98.735, 146.405, 169.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	111.083 - 2.380
Rwork	0.234
R-free	0.24760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ixo
RMSD bond length	0.005
RMSD bond angle	1.264
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	169.640	169.640	2.420
High resolution limit [Å]	2.380	13.040	2.380
Rmerge	0.341	0.070	3.662
Rmeas	0.354	0.073	3.809
Rpim	0.096	0.019	1.043
Number of reflections	99190	714	4890
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	26	23.2	25.7
CC(1/2)	0.998	0.999	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.2 M ammonium citrate pH 7.0 20 % w/v PEG 3350