Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.999990 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.735, 146.405, 169.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.083 - 2.380 |
| Rwork | 0.234 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.264 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 169.640 | 169.640 | 2.420 |
| High resolution limit [Å] | 2.380 | 13.040 | 2.380 |
| Rmerge | 0.341 | 0.070 | 3.662 |
| Rmeas | 0.354 | 0.073 | 3.809 |
| Rpim | 0.096 | 0.019 | 1.043 |
| Number of reflections | 99190 | 714 | 4890 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 26 | 23.2 | 25.7 |
| CC(1/2) | 0.998 | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.2 M ammonium citrate pH 7.0 20 % w/v PEG 3350 |
