7OGF
GTPase HRAS mutant D33K under 900 MPa pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.374 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.418, 38.418, 165.404 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.130 - 1.800 |
| R-factor | 0.247 |
| Rwork | 0.242 |
| R-free | 0.33300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5P21 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.060 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.135 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.521 | |
| Number of reflections | 12669 | 1816 |
| <I/σ(I)> | 10.4 | 1.4 |
| Completeness [%] | 92.5 | 93.2 |
| Redundancy | 5.7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |
