7OGF
GTPase HRAS mutant D33K under 900 MPa pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-07-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.374 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 38.418, 38.418, 165.404 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.130 - 1.800 |
R-factor | 0.247 |
Rwork | 0.242 |
R-free | 0.33300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 5P21 |
RMSD bond length | 0.016 |
RMSD bond angle | 2.060 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.135 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.521 | |
Number of reflections | 12669 | 1816 |
<I/σ(I)> | 10.4 | 1.4 |
Completeness [%] | 92.5 | 93.2 |
Redundancy | 5.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |