7OGD
GTPase HRAS mutant D33K under ambient pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-07-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.374 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 39.787, 39.787, 161.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.184 |
Rwork | 0.181 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 5P21 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.842 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.457 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.846 | |
Number of reflections | 9920 | 1426 |
<I/σ(I)> | 8.8 | 0.8 |
Completeness [%] | 87.9 | 88.7 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |