7OGD
GTPase HRAS mutant D33K under ambient pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.374 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.787, 39.787, 161.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5P21 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.842 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.457 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.846 | |
| Number of reflections | 9920 | 1426 |
| <I/σ(I)> | 8.8 | 0.8 |
| Completeness [%] | 87.9 | 88.7 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |
