7OGC
GTPase HRAS under 650 MPa pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.374 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 38.897, 38.897, 165.088 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5P21 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.997 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.272 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 1.026 | |
| Number of reflections | 16670 | 2353 |
| <I/σ(I)> | 9.8 | 0.4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |
