7OGC
GTPase HRAS under 650 MPa pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-07-23 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.374 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 38.897, 38.897, 165.088 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.700 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 5P21 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.997 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.272 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 1.026 | |
Number of reflections | 16670 | 2353 |
<I/σ(I)> | 9.8 | 0.4 |
Completeness [%] | 99.0 | 99 |
Redundancy | 4.5 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |