7OG9
GTPase HRAS under ambient pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID09 |
Synchrotron site | ESRF |
Beamline | ID09 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2015-07-02 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 0.415 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 39.754, 39.754, 161.798 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.750 |
R-factor | 0.161 |
Rwork | 0.158 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 5P21 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.048 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.22) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.933 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.701 | |
Number of reflections | 15666 | 2243 |
<I/σ(I)> | 13.9 | 1.1 |
Completeness [%] | 99.4 | 99.9 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |