7OG8
Wild-type Hfq protein from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-19 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 6 |
| Unit cell lengths | 60.539, 60.539, 27.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.350 - 1.400 |
| R-factor | 0.2477 |
| Rwork | 0.246 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pno |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.703 |
| Data reduction software | DIALS (2.2.5) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.270 | 30.270 | 1.420 |
| High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
| Rmerge | 0.113 | 0.082 | 1.205 |
| Rmeas | 0.116 | 0.085 | 1.242 |
| Rpim | 0.027 | 0.020 | 0.301 |
| Total number of observations | 211386 | 1489 | 9630 |
| Number of reflections | 11543 | 85 | 569 |
| <I/σ(I)> | 13.7 | 38 | 2.2 |
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 18.3 | 17.5 | 16.9 |
| CC(1/2) | 0.999 | 0.997 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | PEG3350, sodium nitrate, glycerol, bis-tris propane |
