7OG3
IRED-88
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.856, 75.746, 97.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.720 - 1.904 |
| R-factor | 0.2073 |
| Rwork | 0.206 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zgy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.880 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.720 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.286 | |
| Number of reflections | 43308 | 4218 |
| <I/σ(I)> | 9.44 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 0.1M HEPES, 1.39M Sodium citrate tribasic dihydrate |
