7OG3
IRED-88
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.856, 75.746, 97.081 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.720 - 1.904 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zgy |
RMSD bond length | 0.008 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.720 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.286 | |
Number of reflections | 43308 | 4218 |
<I/σ(I)> | 9.44 | |
Completeness [%] | 99.9 | |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.894 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | 0.1M HEPES, 1.39M Sodium citrate tribasic dihydrate |