7OG2

Crystal structure of Pseudoalteromonas luteoviolacea L-amino acid oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96862
Spacegroup name	I 2 2 2
Unit cell lengths	92.045, 134.159, 251.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.170 - 2.800
R-factor	0.2059
Rwork	0.203
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7c4k
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	2.920
High resolution limit [Å]	2.800	2.800
Rpim	0.053	0.387
Number of reflections	38863	4684
<I/σ(I)>	8.3	2
Completeness [%]	100.0
Redundancy	3.4
CC(1/2)	0.995	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.1 M Tris pH 7-8