7OEZ
Leucine Aminopeptidase A mature enzyme in a complex with leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 154.100, 154.100, 87.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.050 - 2.480 |
R-factor | 0.1595 |
Rwork | 0.157 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rtq |
Data reduction software | APEX |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.050 | 2.569 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.097 | 0.483 |
Number of reflections | 22302 | 2195 |
<I/σ(I)> | 7.08 | |
Completeness [%] | 100.0 | |
Redundancy | 2 | |
CC(1/2) | 0.982 | 0.556 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Citrate, pH =5.0, 3.2M Ammonium Sulphate |