7ODZ
Dictyostelium discoideum dye decolorizing peroxidase DyPA in complex with an activated form of oxygen and veratryl alcohol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.910003 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 140.440, 140.440, 95.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.110 - 1.600 |
R-factor | 0.1517 |
Rwork | 0.151 |
R-free | 0.16820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7o9j |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.640 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.022 | 0.279 |
Number of reflections | 125165 | 20558 |
<I/σ(I)> | 25.13 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 33.26 | 33.48 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 2.4 M Sodium malonate pH 7.0 |