7ODB
Crystal structure of bovine Hsc70(aa1-554)E213A/D214A in complex with triazine-derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.611, 50.221, 86.679 |
| Unit cell angles | 90.00, 100.01, 90.00 |
Refinement procedure
| Resolution | 64.610 - 1.662 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fl9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.567 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.400 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmeas | 0.060 | |
| Number of reflections | 64069 | 8985 |
| <I/σ(I)> | 14.33 | 1.17 |
| Completeness [%] | 97.5 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.999 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | PEG 8000, TMAO. potassium chloride, HEPES-NaOH, glycerol |
