7OCX
Crystal Structure of the PID-3 TOFU-6 RRM domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-11 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.00, 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.980, 68.002, 130.585 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 1.700 |
R-factor | 0.1958 |
Rwork | 0.194 |
R-free | 0.23170 |
Structure solution method | SIRAS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.092 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.680 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.086 | |
Rpim | 0.025 | |
Number of reflections | 43394 | 3808 |
<I/σ(I)> | 16.24 | 0.56 |
Completeness [%] | 97.9 | |
Redundancy | 12.7 | |
CC(1/2) | 0.999 | 0.171 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M Sodium acetate pH 5.0 0.2 M Sodium chloride 17 % PEG 3350 |