7OCL
K1K1, a potent recombinant minimal hepatocyte growth factor/scatter factor mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.953725 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.570, 58.750, 65.940 |
Unit cell angles | 90.00, 108.22, 90.00 |
Refinement procedure
Resolution | 43.290 - 1.800 |
R-factor | 0.1981 |
Rwork | 0.197 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Kringle 1 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.290 | 43.290 | 1.670 |
High resolution limit [Å] | 1.610 | 6.240 | 1.610 |
Rmerge | 0.154 | 0.080 | 2.144 |
Rmeas | 0.172 | 0.090 | 2.392 |
Rpim | 0.074 | 0.040 | 1.048 |
Total number of observations | 224081 | 3805 | 21599 |
Number of reflections | 42914 | 781 | 4179 |
<I/σ(I)> | 4.6 | 11.9 | 0.6 |
Completeness [%] | 99.9 | 99.3 | 99.8 |
Redundancy | 5.2 | 4.9 | 5.2 |
CC(1/2) | 0.986 | 0.928 | 0.454 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290.15 | 100 mM Tris/Bicine pH 8.5, 30 mM Sodium nitrate, 30 mM Sodium phosphate dibasic, 30 mM Ammonium sulfate, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD |