7OBZ

Structure of RsLOV D109G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	90.960, 65.800, 122.720
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	45.370 - 2.000
R-factor	0.2188
Rwork	0.217
R-free	0.26340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hia
RMSD bond length	0.007
RMSD bond angle	0.986
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.000	2.000
Rmeas	0.088	3.440
Number of reflections	97821	15597
<I/σ(I)>	9.32
Completeness [%]	99.6	98.9
Redundancy	3.72
CC(1/2)	0.999	0.189

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	293	0.1 M HEPES/NaOH pH7.4; 3.4 M NaCl; 0.02 M di-spermidine-triphosphat