7OBJ

Carbon regulatory PII-like protein SbtB from Synechocystis sp. 6803 in complex with cyclic di-AMP (c-di-AMP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.000
Spacegroup name	P 32
Unit cell lengths	63.171, 63.171, 81.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.740 - 2.000
R-factor	0.1797
Rwork	0.178
R-free	0.21500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5o3r
RMSD bond length	0.017
RMSD bond angle	1.927
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.740	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.064	2.231
Number of reflections	24685	3942
<I/σ(I)>	20.61	1.03
Completeness [%]	99.7
Redundancy	10.3	10.1
CC(1/2)	1.000	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1M phosphate-citrate pH 4.2, 40% (w/v) PEG 300