7OBH
Crystal structure of 14-3-3 sigma in complex with NPM1 phosphopeptide and stabilizer Fusicoccin-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | RIGAKU MICROMAX-003 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-07 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5419 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.475, 112.195, 62.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.230 - 2.000 |
R-factor | 0.1437 |
Rwork | 0.142 |
R-free | 0.18040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.034 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.230 | 2.040 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.101 | 0.243 |
Number of reflections | 19953 | 979 |
<I/σ(I)> | 13.4 | 6.6 |
Completeness [%] | 100.0 | 99.7 |
Redundancy | 6.3 | 6 |
CC(1/2) | 0.995 | 0.952 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.095 M Hepes pH 7.3, 25%PEG 400, 0.19 M CaCl2 and 5 % Glycerol |