7OBF
Crystal structure of the human VH antibody domain HEL4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.07234 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 76.080, 76.080, 187.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.250 - 2.699 |
R-factor | 0.2166 |
Rwork | 0.214 |
R-free | 0.26370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ohq |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.4) |
Phasing software | Sir2014 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.250 | 48.250 | 2.830 |
High resolution limit [Å] | 2.699 | 8.950 | 2.700 |
Rmerge | 0.233 | 0.154 | 0.840 |
Rmeas | 0.255 | 0.172 | 0.949 |
Rpim | 0.099 | 0.072 | 0.432 |
Total number of observations | 1361 | 4407 | |
Number of reflections | 7840 | 242 | 1009 |
<I/σ(I)> | 8.1 | 14 | 2.9 |
Completeness [%] | 99.1 | 96 | 97.5 |
Redundancy | 6.3 | 5.6 | 4.4 |
CC(1/2) | 0.968 | 0.949 | 0.711 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | MgSO4, Hepes, glycerol |