Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.580, 103.010, 95.060 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 34.840 - 3.000 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wao |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18_3861) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.840 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 | 0.240 |
| Number of reflections | 19742 | 1982 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1 M HEPES, PH 7.0, 50 % PEG 4000 |
