Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.220, 154.330, 28.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.443 - 1.500 |
| Rwork | 0.152 |
| R-free | 0.18600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zbp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.443 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.443 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rpim | 0.013 | 0.150 |
| Number of reflections | 51783 | 3353 |
| <I/σ(I)> | 28.1 | 6 |
| Completeness [%] | 99.4 | 93.7 |
| Redundancy | 11.8 | |
| CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate pH 6.5, 30 % w/v PEG 8000 |
